| HEPES Chemical Properties |
| Melting point | 234-238 °C |
| density | 1.07 g/mL at 20 °C |
| refractive index | n 20/D 1.339 |
| storage temp. | 2-8°C |
| solubility | H 2 O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| PH Range | 7.0 - 7.6 |
| pka | 7.5(at 25ºC) |
| Water Solubility | Soluble |
| λmax |
λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
| Safety Information |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-22-36-26 |
| WGK Germany | 1 |
| RTECS | TL6809000 |
| TSCA | Yes |
| HS Code | 29335995 |
| MSDS Information |
| HEPES Usage And Synthesis |
| Chemical Properties | White crystalline powder |
| Uses | Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. |
| Uses | Biological buffer. |
| Definition | ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. |
| Biological Activity | Multi purpose buffer used in biological research. |
| Purification Methods | Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] |